UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI N-HEKSAN, FRAKSI ETIL ASETAT, FRAKSI AIR DARI BIJI BUAH PEPAYA (Carica papaya L.) TERHADAP Escherhia coli ATCC 25922 SECARA DIFUSI DAN DILUSI

ABSTRACT

ANTIBACTERIAL ACTIVITY TEST OF EXTRACT AND N-HEXAN
FRACTION, ETHYLE ACETATE FRACTION, WATER FRACTION
FROM PAPAYA FRUIT SEEDS (Carica papaya L.) AGAINST
Escherichia coli ATCC 25922 BY DIFFUSION

AND DILUTION

Baiq Mitasari

This study aims to determine whether the ethanol extract and n-hexane
fraction, ethyl acetate fraction and water fraction of papaya fruit seeds (Carica papaya
L.) have antibacterial activity against Escherichia coli ATCC 25922 using diffusion
and dilution methods. Sampling of papaya seeds was carried out in the yard of a
house in Purworejo, Central Java. The data obtained were analyzed using SPSS
(Statistical Product for Service Solutions) software using the One Way Anova
(Analysis of Variance) test. The existence of a significant difference in the test was
indicated by the p value kurang lebih 0.05. The concentrations used in this test are 1%, 5%, 10%, 15%, 20%, 25%. Positive controls used were ciprofloxacin and 1% DMSO as
negative controls. The results of this study were 96% ethanol extract, n-hexane, ethyl acetate and water
fractions from papaya fruit seeds (Carica papaya L.) had antibacterial activity against
the growth of Escherichia coli ATCC 25922. Ethyl acetate fraction from ethanol
extract of papaya seeds ( Carica papaya L.) is the most active fraction as an
antibacterial against Escherichia coli ATCC 25922. The value of the Minimum
Inhibitory Concentration (MIC) is 25% and the value of the Minimum Kill
Concentration (KBM) obtained is 25%.