Penetapan Kadar Total Flavonoid Dan Aktivitas Antioksidan Ekstrak Etanol Batang Dan Daun Turi Putih (Sesbania grandiflora L) Dengan Metode ABTS

DEVI, SILVIA (2022) Penetapan Kadar Total Flavonoid Dan Aktivitas Antioksidan Ekstrak Etanol Batang Dan Daun Turi Putih (Sesbania grandiflora L) Dengan Metode ABTS. Other thesis, Universitas Duta Bangsa Surakarta.

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Abstract

Turi putih (Sesbania grandiflora L) merupakan tanaman yang termasuk dalam famili Fabaceae. Turi putih (Sesbania grandiflora L) mengandung banyak senyawa metabolit sekunder meliputi alkaloid, saponin, tanin, steroid, triterpenoid, fenolik dan flavonoid (Nista et al., 2010). Namun dalam kalangan masyarakat pemanfaatan turi putih (Sesbania grandiflora L) digunakan sebagai tanaman hias dan bunga turi putih (Sesbania grandiflora L) sebagai bahan makanan, turi putih (Sesbania grandiflora L) juga memiliki manfaat lain yaitu sebagai antioksidan. Tujuan penelitian ini yaitu untuk mengetahui kadar flavonoid dan aktivitas antioksidan batang dan daun turi putih (Sesbania grandiflora L) yang diekstraksi dengan menggunakan pelarut etanol 95%. Penetapan kadar flavonoid dengan menggunakan metode kolorimetri dengan kontrol positif yaitu kuersetin. Pengujian aktivitas antioksidan dengan menggunakan metode ABTS. Hasil penelitian menunjukkan bahwa kadar flavonoid total ekstrak etanol batang turi putih (Sesbania grandiflora L) sebesar 12,08 mg/L dan kadar flavonoid total ekstrak etanol daun turi putih (Sesbania grandiflora L) sebesar 11,28 mg/L. Serta ekstrak etanol batang, daun dan kombinasi ekstrak etanol batang dan daun turi putih (Sesbania grandiflora L) memiliki aktivitas antioksidan dengan nilai IC50 berturut-turut sebesar 36,519 mg/L, 37,438 mg/L, dan 40,860 mg/L.

White turi (Sesbania grandiflora L) is a plant that belongs to the Fabaceae family. White turi (Sesbania grandiflora L) contains many secondary metabolites including alkaloids, saponins, tannins, steroids, triterpenoids, phenolics and flavonoids (Nista et al., 2010). However, in the community the use of white turi (Sesbania grandiflora L) is used as an ornamental plant and white turi flowers (Sesbania grandiflora L) as food, white turi (Sesbania grandiflora L) also has other benefits, namely as an antioxidant. The purpose of this study was to determine the levels of flavonoids and antioxidant activity of white turi (Sesbania grandiflora L) stems and leaves extracted using 95% ethanol as solvent. Determination of flavonoid levels using colorimetric method with positive control, namely quercetin. Testing of antioxidant activity using the ABTS method. The results showed that the total flavonoid content of the ethanol extract of the white turi stem (Sesbania grandiflora L) was 12.08 mg/L and the total flavonoid content of the ethanol extract of the white turi leaf (Sesbania grandiflora L) was 11.28 mg/L. As well as ethanol extract of stems, leaves and a combination of ethanolic extracts of stems and leaves of white turi (Sesbania grandiflora L) have antioxidant activity with IC50 successively of 36,519 mg/L, 37,438 mg/L, and 40,860 mg/L.

Item Type: Thesis (Other)
Uncontrolled Keywords: Kata Kunci : Turi putih (Sesbania grandiflora L), Antioksidan, ABTS Keywords: white turi (Sesbania grandiflora L), antioxidant, ABTS
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Ilmu Kesehatan > S1 - Farmasi
SWORD Depositor: Perpustakaan Fikes
Depositing User: Perpustakaan Fikes
Date Deposited: 20 Oct 2022 07:15
Last Modified: 20 Oct 2022 07:15
URI: https://eprints.udb.ac.id/id/eprint/1499

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