UJI DIURETIK INFUSA DAUN TEH HIJAU (CAMELLIA SINENSIS, l.) PADA TIKUS JANTAN GALUR WISTAR

ABSTRACT

GREEN TEA LEAF INFUSION DIURETIC TEST (CAMELLIA SINENSIS L) ON

MALE WHITE RATS SITAR STRAIN

Fadista Kurnia Dewi

Green tea leaves are natural materials, contains flavonoids can increase the volume
of urine, This study aims to determine the diuretic effect of ethanol extract of green tea
leaves in male Swiss mice.
This study is an experimental post control group design using in male swiss mice
were divided into 5 groups: group I negative control with CMC Na 0,5%, group II positive
control with furosemide dose of 3,6mg/KgBB, and group III ethanol extract of green tea
leaves dose of 250 mg/gBB, group IV ethanol extract of green tea leaves dose of 500
mg/gBB, group V ethanol extract of green tea leaves dose of 750 mg/gBB. Before
treatment all mice were given warm water orally, then give it according to the group. Tests
on the diuretic effect is done by measuring the urine volume, the measurements of to be
conducted every 60 minutes for 6 hours. Data analysis was performed using SPSS 23 with
One-way ANOVA test and continue LSD.
Results of the average cumulative urine volume group I of 7,01 ml, group II of
12,98 ml, group III of 9, 13 ml, gruop IV of 12, 57 ml, and gruop V of 12, 81 ml.
Furthermore, analysis of data using SPSS. LSD test results showed that the group V differ
significantly with group II, ie with significant value 0,850 (p lebih besar 0.05). Based on the average
volume and SPSS can be concluded that the ethanol extract of green tea leaves have a
diuretic.